R5; Background Poly(ADP-dbose) polymerase (PARP) plays an important role in cell survival and death. However, the mechanisms involved are not fully understood. Therefore, we investigated the effect of inhibition of PARP on acute myocardial infarction (AMI) at different time points in rats.Methods AMI was induced in rats by ligating the left antedor descending coronary artery. One group received 3-aminobenzamide (3-AB, a kind of PARP inhibitor) (30 mg/kg) by intrapedtoneal injection. The changes of ultramicrostructure of cardiocytes in infarction region were noted, PARP cleavage was measured by Westem blotting,and expressions of protein of PARP and apoptosis inducing factor (AIF) were measured by immunohistochemical staining after treatment with 3-AB for 2 hours, 4 hours, 6 hours, 1 week, 4 weeks and 8 weeks. Results Few damages to the ultramicrostructure of cardiocytes were observed after treatment with 3-AB. PARP cleavage was detected as eady as 4 hours and markedly increased by 6 hours following AMI without

Chinese medical journal

3-aminobenzamide; poly(ADP-ribose) polymerase; apoptosis inducing factor; apoptosis; DNA repair; acute myocardial infarction