A novel gold-label silver-stain electrochemical immunosensor based on polythionine-gold nanoparticles (PTh-Au NPs) modified glassy carbon electrode (GCE) as a platform and secondary antibody labeled Au NPs (Ab2-Au NPs) as immumoprobe for carcinoembryonic antigen (CEA) detection. The sandwich-type biosensor adopted anodic stripping voltammetry to detect silver stripping signal when the Ab2-Au NPs of the formed immunocomplexes were stained with silver. [Display omitted] A novel gold-label silver-stain electrochemical immunosensor was developed based on polythionine-gold nanoparticles (PTh-Au) modified glassy carbon electrode (GCE) as a platform and secondary antibody labeled Au NPs (Ab2-Au) as immumoprobe for carcinoembryonic antigen (CEA) detection. The sandwich-type biosensor adopted anodic stripping voltammetry to detect silver stripping signal when the Ab2-Au of the formed immunocomplexes were stained with silver. The optimized detection conditions were investigated. The effect of different electrochemical responses at various concentrations of CEA was checked by anodic stripping voltammetry. This immunosensor showed a low detection limit of 0.055 ng/mL and a wide linear calibration of 0.1–120 ng/mL (R2 = 0.99856). Moreover, this immunoassay also existed the advantages of good reproducibility, stability and selectivity. Thus, this immunosensing protocol may provide a potential application for effective clinical detection of CEA.

Chinese chemical letters

Carcinoembryonic antigen; Electrochemical immunosensor; Sandwich-type immunoassay; Gold-label silver-stain; Anodic stripping voltammetry; Carcinoembryonic antigen; Electrochemical immunosensor; Sandwich-type immunoassay; Gold-label silver-stain; Anodic stripping voltammetry

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