A new alpha-fetoprotein-MIP (AFP-MIP) immunosensor based on glass carbon electrode (GCE) modified with polythionine (PTh) and gold nanoparticles (AuNPs) was successfully prepared for the sensitive detection of AFP. The AFP-MIP immunosensor presented a facile preparation, low sample consumption, and good stability, and could become a new promising method for the detection of AFP. [Display omitted] A new α-fetoprotein-MIP (AFP-MIP) immunosensor based on glass carbon electrode (GCE) modified with polythionine (PTh) and gold nanoparticles (AuNPs) was successfully fabricated for sensitive detection of α-fetoprotein (AFP). Through controlling electropolymerization, A “polydopamine (PDA)-AFP” complex was achieved applying AFP as template and dopamine (DA) as imprinted monomers. After elution, the specific cavities can adsorb the target molecules. Using differential pulse voltammetry (DPV) detection, the peak current decreased with the increase in concentration of AFP, and the linear response range of the AFP-MIP immunosensor was from 0.001 ng/mL to 800 ng/mL with the detection limit as low as 0.8138 pg/mL. The MIP immunosensor could become a new promising method for the detection of AFP. Furthermore, this MIP sensor was demonstrated in testing AFP in human serum samples with satisfactory results.

Chinese chemical letters

α-Fetoprotein; Molecular imprinting polymer; Electropolymerization; Differential pulse voltammetry; Immunosensor; α-Fetoprotein; Molecular imprinting polymer; Electropolymerization; Differential pulse voltammetry; Immunosensor

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